Applications
Envue's technology offers a unique tool for optical, label-free characterization of objects smaller than any other
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Study proteins and other biomolecules
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Mass and Size characterization
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NSM makes it is possible to identify and track smaller objects than withany with other label-free optical technique on the market.
Upon identification, our unique software extracts both the mass and size of individual objects in the same measurement. This can be used to identify components in your sample that would be invisible to conventional optical techniques. At the same time NSM is capable of separating objects with different shape or density which is impossible for techniques that only measure size or mass.
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DNA tracking and characterization
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Because NSM utilizes nanochannels, large molecules such as DNA will be partially stretched when they move through the channel. The high sensitivity enables studies of DNA without the need for fluorescent tagging that could affect the molecules functionality or stability.
Molecular interactions
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See molecular interactions with your own eyes! Analyze the number of occurrences and their duration to determine binding constants or study molecule-substrate affinity and molecule-molecule interactions.
Size and mass histogram of three protein samples. Note how three subpopulations of Ferritin are clearly identified from their mass although they have the same apparent radius. This stems from Ferritins ability to store different amounts of iron, resulting in molecules of varying mass but the same size.
Video of a lambda-DNA moving through a nanochannel
Kymograph showing a timelapse of two Ferritin molecules merging into one
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Use it for quality control
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Identifying species in complex media
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The high size resolution of NSM allows identification of subpopulations that are invisible to other commonly used techniques. For example, extracellular vesicles in cell media can be detected and clearly separated from the complex background consisting of a large number of lipoproteins.
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Oligomer formations
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The function of nanoparticles and molecules can change dramatically if aggregation occurs. Use our microscope and nanofluidic chips to see if your sample tends to form dimer, trimer or other oligomer aggregates.
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Particle counting
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Count the number of nanoparticles in your sample. By letting the sample flow through our nanochannels one can count every nanoparticle in the liquid, determine the size and optical contrast.
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